KAER LABS
3D ORGAN IMAGING
Vascular Biology
CIRCLE OF WILLIS ANATOMY
Aim: Understand the prevalence of intracranial aneurysm in mutated mouse models
Approach: Absorbing contrast agent injected in mouse to improve contrast of the circle of Willis. Fresh frozen mouse brain, label free
Result: Vessel detection and segmentation
Figure: Autofluorescence signal of a paraffin embedded brain of a control mouse (A) and of a mouse injected with the vascular absorbing agent (B). 2D Segmentation of the vascular network from the image shown in B was performed throughout the full stack (C) and used to generate a maximum projection of the 3D reconstructed full brain vascular network (D). Scale bar 1mm
Video: animation of the 3D reconstructed full brain vascular network obtained with a maximum projection approach
Courtesy of Micropicell / Anne Clémence Vion, Céline Menguy, Nantes University, Nantes, France
Kratoscopy as a service​
In collaboration with the microscopy and histology platform MicroPICell from Nantes University / SFR François Bonamy, Kaer Labs can propose the technique as a service. It is as simple as shipping us your organs embedded in paraffin or frozen in OCT. Contact us for more information on prices and processes.
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