KAER LABS
CANCER
Theranostic imaging of dual labelled antibody in glioblastoma models
GLIOBLASTOMA
Aim: The study evaluates a novel preclinical bimodal imaging agent for glioblastoma (GBM), aimed at guiding and facilitating complete tumor resection during surgery. Enhancing the effectiveness of Fluorescence-Guided Surgery (FGS) requires precise co-localization of the PET and fluorescence signals of the agent. To confirm the correlation between these two distinct signals, an ex vivo analysis was performed.
Approach: Brain from a mice bearing orthotopic human GBM was harvested and frozen 48hr post injection of the bimodal imaging agent. Serial sectioning of the tissue was carried out and tumor fluorescence was imaged with a 785nm excitation. Individual 2D sections were collected in the region of the tumor and imaged by autoradiography.
Figure: Ex vivo co-localization of the fluorescent and nuclear signals in the brain tumor 48h p.i. of the biomodal imaging agent. A. Comparison of the 3D fluorescence reconstruction using the kratoscope (Kaer Labs) and the sagittal PET nuclear imaging. B. Comparison of nuclear and fluorescent signal on corresponding brain tumor sections obtained by autoradiography and block face fluorescence imaging (scale bars represent 2.4 mm). (Adapted from Hautiere et al. 2024).
Results: Correlation of the two imaging modalities show co-localization of the fluorescent and nuclear signals. These results validate the use of this bimodal agent as a promising tool for the diagnostic and FGS of glioblastoma.
Publication: Hautiere, Marie et al. “Preoperative PET imaging and fluorescence-guided surgery of human glioblastoma using dual-labeled antibody targeting ETA receptors in a preclinical mouse model: A theranostic approach.” Theranostics vol. 14,16 6268-6280. 30 Sep. 2024, doi:10.7150/thno.98163
Kratoscopy as a service​
In collaboration with the microscopy and histology platform MicroPICell from Nantes University / SFR François Bonamy, Kaer Labs can propose the technique as a service. It is as simple as shipping us your organs embedded in paraffin or frozen in OCT. Contact us for more information on prices and processes.
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